BIO805 Protein Research Methods

[1–6, 3 cr.]

This course is a laboratory course in molecular biology.  The course  aims  at familiarizing students with the basic techniques currently used in this field, and supplies them with the necessary tools to carry on independent research that is needed for their Master’s  Thesis. Techniques include protein isolation and purification, protein handling and quantification, gel electrophoresis, western immunoblot, ELISA, column chromatography (gel filtration, ion exchange chromatography, and affinity chromatography), HPLC, GCMS, NMR, and lipoprotein separation, using density gradient ultracentrifugation, and other advanced laboratory techniques in protein research.